<i>Pig-L</i> mediates virulence, biofilm formation, and oxidative stress tolerance in <i>Clostridioides difficile</i>.

ABSTRACT:

Background

Clostridioides difficile infection (CDI) represents a significant global public health concern. The Phosphatidylinositol Glycan Class L (pig-L) gene in C. difficile encodes an enzyme critical for the biosynthesis of Glycosylphosphatidylinositol (GPI) anchor, which play a vital role in bacterial surface protein localization and function.

Methods

To investigate the role of pig-L in C. difficile pathogenesis, we utilized CRISPR-Cas9 gene editing to generate a pig-L knockout strain and a complementation strain in the wild-type (WT) background. Phenotypic characterization of these strains was performed through a suite of assays, including virulence assays, biofilm formation assays, oxidative stress sensitivity testing, and anti

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC12613277 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature