CDK5 regulates PPARγ/NF-κB signaling to exacerbate obesity-related osteoarthritis via modulating macrophage polarization and chondrocyte apoptosis.

ABSTRACT:

Background

Obesity is an important risk factor for osteoarthritis (OA), but the mechanisms associated with OA progression are still not fully understood. The aim of this study was to investigate the role of cyclin-dependent kinase 5 (CDK5) in regulating the peroxisome proliferator-activated receptor gamma (PPARγ)/nuclear factor-κB (NF-κB) signaling pathway and its effect on obesity-related OA.

Methods

By analyzing tissue samples from obese and nonobese patients with OA in conjunction with a high-fat diet (HFD)-induced obese mouse model of OA, we investigated the expression level of CDK5 and its effects on inflammation and apoptosis. The role of CDK5 in macrophage polarization and chondrocyte apoptosis was further explored by gene knockdown and pharmacological intervention.

SUBMITTER: Li Y 

PROVIDER: S-EPMC12613357 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature