Integrated transcriptomic and WGCNA identify drought-responsive TFs in Larix olgensis.

ABSTRACT: Global forests face increasingly severe drought events, which suppress the growth of larch. Transcription factors (TFs), acting as regulators, can increase plant stress tolerance by modulating gene expression networks. This study integrated RNA-seq, WGCNA and physiology to systematically analyze the adaptation mechanisms of Larix olgensis under drought stress. Among the 50,110 unigenes identified via RNA-seq, 1,391 unigenes were annotated as TFs. These TFs were clustered into four distinct clusters based on expression profiles. TFs within Cluster 1 exhibited significant differential expression during visible wilting of L. olgensis (72 h and 96 h). Ten TFs were selected as candidate TFs from Cluster 1 based on fold-change rank (p < 0.05). Subsequent WGCNA clustered the 50,110 unigenes into

SUBMITTER: Zhao W 

PROVIDER: S-EPMC12613604 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature