All-Aqueous Phase Segregation Integrated Electrochemical Aptamer Biosensor Enables Picomolar Detection of SARS-CoV-2 Spike Protein.

ABSTRACT: Electrochemical aptamer biosensors (E-ABs) are promising tools for rapid point-of-care (POC) diagnosis which utilize aptamers for the molecular recognition of specific disease biomarkers. However, E-AB sensitivity is typically limited by the binding affinity (K_D) of the aptamer (often in the micromolar-nanomolar range), while biomarker concentrations in biofluids are often at the picomolar level or below. Liquid-liquid phase separation (LLPS) provides a robust framework to concentrate the target biomarker based on affinity-controlled partitioning within an all-aqueous environment. In this study, the integration of LLPS into E-AB is reported to overcome the sensitivity limitation and achieve picomolar Severe acute respiratory syndroome coronavirus 2 spike protein (SARS-CoV-2 S pr

SUBMITTER: Siu RH 

PROVIDER: S-EPMC12614154 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature