A high-throughput <i>N</i>-glycan analysis strategy with targeted mass spectrometry (HTnGQs-target) for liver disease diagnosis.

ABSTRACT: Hepatocellular carcinoma (HCC), a global leading cause of cancer-related mortality, is critically hindered by delayed diagnosis due to the lack of sensitive early biomarkers. Alterations in N-glycan composition and structure, which are closely associated with HCC pathogenesis, hold great promise for early detection; however, conventional mass spectrometry-based glycomic methods are limited by low sample throughput. To address this, we developed HTnGQs-target-a high-throughput targeted mass spectrometry approach integrating methylamine derivatization and 6-plex aminoxy TMT labeling. This approach enables robust quantitative analysis of up to 144 serum samples per day with high sensitivity and reproducibility. Applied to a cohort of 320 serum samples encompassing the full spectrum of

SUBMITTER: Liu X 

PROVIDER: S-EPMC12614178 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature