Controlled Reassociation of Multistranded, Polycrossover DNA Molecules into Double Helices.

ABSTRACT: Shape-changing DNA nanostructures have found applications in biosensing, drug delivery, and data storage. Here, we use sequence and temperature-controlled reassociation of one type of a DNA nanostructure (paranemic crossover (PX) DNA) into another structure (duplex). In the presence of an anti-PX structure that is composed of strands that are each complementary to those in PX DNA, the structures reassociate at specific temperatures to form duplexes. Using the denaturing agent formamide, we decreased the temperature required for this reassociation. We demonstrate tunable biostability, where the structures before and after reassociation show vastly different nuclease resistance against DNase I. We further extend the strategy to other polycrossover DNA molecules such as a double crossover mot

SUBMITTER: Kabbara N 

PROVIDER: S-EPMC12614193 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature