Exploration of Yeast Species Suitable for Preparation of Stable Isotope-Labeled Internal Standards Extracts (SILIS).

ABSTRACT: Isotope dilution mass spectrometry is a widely used method for measuring intracellular metabolite concentrations, relying on the ratio of peak areas between the target compound and its stable isotope-labeled internal standard. For metabolome analysis of microorganisms, comprehensive concentration measurements have been achieved through the preparation of stable isotope-labeled internal standard extracts (SILIS). Methods have been developed to prepare SILIS by extracting crude metabolites from fully ¹³C-labeled bacteria Escherichia coli and yeasts Saccharomyces cerevisiae and Pichia pastoris (Komagataella phaffii). For cost-effective preparation of SILIS, ideal characteristics of host yeasts include rapid cell growth, high biomass production, and signi

SUBMITTER: Seike T 

PROVIDER: S-EPMC12614885 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature