Detection of lymphoproliferative disease virus in domestic and wild turkeys through RNAscope in situ hybridization and immunohistochemistry.

ABSTRACT: Diagnosis of lymphoid hyperplasia and neoplasia due to lymphoproliferative disease virus (LPDV; Retroviridae, Alpharetrovirus) infection in turkeys is challenging due to histopathologic similarities with nonspecific inflammation and cellular responses to other retroviral infections. Localization of LPDV RNA or protein antigens within affected tissues, which has previously not been shown, would allow for more definitive diagnoses. We evaluated formalin-fixed paraffin-embedded tissues from 32 turkeys, including 15 naturally infected wild turkeys, 11 experimentally infected domestic turkeys, and 6 uninfected wild and domestic turkeys, using RNAscope in situ hybridization (ISH) and immunohistochemistry (IHC). ISH probes targeted a segment of the gag gene, and IHC antibodie

SUBMITTER: Goodwin CC 

PROVIDER: S-EPMC12614898 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature