Monkeypox Virus Transcriptional Profiles and Host Responses in Skin Lesion Swabs Among Individuals With Human Immunodeficiency Virus.

ABSTRACT:

Background

Mpox disease, caused by the monkeypox virus (MPXV), remains a global health concern with nearly half of all cases occurring among individuals with human immunodeficiency virus (HIV). While recent studies have advanced our understanding of poxvirus pathogenesis, the molecular effects of mpox and HIV coinfection are still poorly understood. This study uses dual RNA sequencing (RNA-seq) to characterize host and viral gene expression in skin lesion swabs from people with mpox, including individuals with and without HIV.

Methods

Our cohort included 19 participants with confirmed MPXV infection, with 53 total skin lesion swabs collected during the early (7-13 days post-symptom onset) and late (15-21 days post-symptom onset) stages of mpox disease. RNA-seq was used to as

SUBMITTER: Hurst JR 

PROVIDER: S-EPMC12614975 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature