Deciphering the epigenetic role of KDM4A in pancreatic β-like cell differentiation from iPSCs.

ABSTRACT:

Introduction

Pancreatic β cells derived from human induced pluripotent stem cells (hiPSCs) represent a promising therapeutic avenue in regenerative medicine for diabetes treatment. However, current differentiation protocols lack the specificity and efficiency required to reliably produce fully functional β cells, limiting their clinical applicability. Epigenetic barriers, such as histone modifications, may hinder proper differentiation and the acquisition of essential maturation markers in these cells.

Methods

hiPSCs were cultured under feeder-free conditions and subjected to lentiviral transduction with shRNA constructs to silence KDM4A. Differentiation into pancreatic β-like cells was performed using stepwise protocols, with or without doxycycline supplementation, t

SUBMITTER: Arroyave F 

PROVIDER: S-EPMC12615240 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature