Host factor Rab4b mediates internalization and intoxication of 3D4/21 cells by the active subunit of the <i>Glaesserella parasuis</i> cytolethal distending toxin via influencing EEA1 expression.

ABSTRACT:

Background

The cytolethal distending toxin (CDT), a significant exotoxin, is closely linked to the pathogenicity of Glaesserella parasuis (GPS), but its pathogenic not yet fully elucidated. Previously, we identified Rab4b as a potential host factor contributing to the cytotoxicity of GpCDT through a whole-genome CRISPR/Cas9 screen technology, and subsequently confirmed its association with GpCDT cytotoxicity in PK-15 cells.

Aims

In this study, our data first indicated that Rab4b could interact with the active subunit of the Glaesserella parasuis cytolethal distending toxin.

Methods

Investigating the relationship between Rab4b and GpCDT subunits as confirmed by coimmunoprecipitation assay. Next, the porcine alveolar macrophage cell li

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12615497 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature