Development of Cellular Energy Metabolism During Differentiation of Human iPSCs into Cortical Neurons.

ABSTRACT: Neuronal differentiation requires extensive metabolic remodeling to support increased energetic and biosynthetic demands. Here, we present an integrated multi-omics and functional characterization of metabolic transitions during early differentiation of human induced pluripotent stem cells (iPSCs) into excitatory cortical neurons using doxycycline-inducible overexpression of neurogenin-2 (NGN2). We analyzed parental iPSCs and induced neurons (iNs) at days 7 and 14 of differentiation, integrating gene expression profiling, label-free quantitative proteomics, high-resolution respirometry, fluorescence lifetime imaging microscopy (FLIM), and ¹³C₆-glucose metabolic flux analysis. Our data reveal progressive metabolic remodeling associated with neuronal maturation, including enhanced

SUBMITTER: Danacikova S 

PROVIDER: S-EPMC12615542 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature