Comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells.

ABSTRACT: Chinese hamster lung (CHL)-YN cells are promising novel hosts for producing therapeutic antibodies with the potential to shorten the research, development, and manufacturing timelines in biopharmaceutical production. CHL-YN cells grow twice as fast as Chinese hamster ovary (CHO) cells, with a doubling time of 8.1 h. These cells possess strong glutamine synthetase activity, allowing them to be cultured in glutamine-free media. In this study, we conducted comparative transcriptomics and proteomics among CHL-YN cells, CHO cells, and lung tissue from Chinese hamster to better understand the global characteristics of CHL-YN cells and determine whether these features are tissue-derived or unique to the cell line. Omics profiling revealed that CHL-YN cells, in contrast to CHO cells, exhibit highl

SUBMITTER: Tsunoda Y 

PROVIDER: S-EPMC12615816 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature