Epigenetic regulation of TIPE3 in nasopharyngeal carcinoma and its impact on the hedgehog signaling pathway.

ABSTRACT:

Objective

This study aims to investigate the epigenetic regulation of TIPE3 in nasopharyngeal carcinoma (NPC) and its role in tumor progression, with a focus on enhancer elements and associated histone modifications, as well as the involvement of the Hedgehog signaling pathway.

Methods

The regulation of TIPE3 was explored through chromatin immunoprecipitation (ChIP) assays to identify histone modifications, specifically H3K27ac and H3K4me3, associated with enhancer elements upstream of the TIPE3 promoter. CRISPR-Cas9 technology was employed to delete these enhancer regions in NPC cell lines. The effects on TIPE3 expression, cell proliferation, and metastasis were assessed both in vitro and in vivo. Additionally, the roles of the histone-modifying enzymes KAT2A and SETD7 in m

SUBMITTER: Cheng Z 

PROVIDER: S-EPMC12615883 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature