CD9 antibody enhances the anti-leukemia efficacy of γδT cells in adult B cell acute lymphoblastic leukemia.

ABSTRACT: B cell acute lymphoblastic leukemia (B-ALL) is a hematological malignancy with a heterogeneous prognosis. Using single-cell RNA sequencing (scRNA-seq), we profiled 10 samples from three relapsed patients, three newly diagnosed patients, and four healthy volunteers. We identified a preB_CD9 cluster with predominant S-phase distribution (53.5%), enhanced communication with leukemic clusters, and reduced interaction with immune cells, which were the most pronounced changes observed during the progression of adult B-ALL. CD9 was overexpressed in relapsed patients, validated as a poor prognostic marker in the GSE34941 dataset. Relapsed cases showed immunosuppressive tumor microenvironments (TMEs) with diminished γδT cells, while the preB_CD9 cluster expressed γδT cell-targe

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12616032 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature