Macrophage Phospholipase D3 promotes atherosclerosis via exacerbating foam cell formation and inducing inflammatory responses.

ABSTRACT:

Background

Atherosclerosis is a chronic inflammatory disease and a major cause of global morbidity and mortality. Phospholipase D3 (PLD3) has been reported to be elevated in atherosclerotic plaques, yet its functional role and molecular mechanisms remain unclear. This study investigated the role of PLD3 in atherosclerosis.

Methods

Single-cell RNA sequencing of human atherosclerotic tissues was analyzed to define PLD3 expression. Validation was performed in ApoE^-/- mice and THP-1-derived foam cells by qRT-PCR and western blotting. Lentiviral-mediated PLD3 knockdown was followed by oxidized LDL (ox-LDL) stimulation. Lipid accumulation and uptake were assessed by Oil Red O, BODIPY, and DiI-ox-LDL assays, while inflammatory cytokines were quantified by qRT-PCR. RNA sequencing w

SUBMITTER: Li T 

PROVIDER: S-EPMC12616045 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature