Microbiome-based profiles of airborne bacteria to support microbial risk assessment in cleanroom environments.

ABSTRACT:

Introduction

Maintaining aseptic conditions is essential for cell product processing, as sterilization cannot be applied to living cells. Conventional environmental monitoring relies on particle counts and culture-based colony-forming unit measurements. These indicators fail to capture much of the diversity and provenance of airborne microbes because many taxa are nonculturable or require growth conditions not supported by standard culture media. Therefore, comprehensive DNA-based microbiome analysis is critical for evaluating microbial risks that conventional methods may overlook; however, such studies remain limited in cleanroom settings. This study aimed to comprehensively visualize the structure of airborne microbial communities in cleanroom environments and clarify microbial r

SUBMITTER: Mizuno M 

PROVIDER: S-EPMC12616117 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature