Fluid shear stress-mediated upregulated exosomal-IGF2 promotes hepatocellular carcinoma cell migration and metastasis.

ABSTRACT: Exosomes, as important intercellular message transporters, can be secreted by hepatocellular carcinoma (HCC) cells and transported to adjacent cells, thus promoting their migration and invasion in turn. However, whether the exosomes secreted by HCC are affected by physical abnormalities, such as fluid shear stress (FSS), is still largely unknown. Here, we observed that 1.4 dyn/cm² FSS could significantly increase the release of exosomes by up-regulating Rab27a and down-regulating Rab7 in HCC cells. Exosomes from FSS-induced HCC cells were more effective at encouraging recipient cell migration and invasion. Exosomes produced by static or FSS-stimulated cells were thoroughly analyzed using quantitative proteomics, and more than 1000 exosome proteins were found. Based on the differ

SUBMITTER: Feng T 

PROVIDER: S-EPMC12616160 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature