Stereoisomeric Separation of Flavonoids by Two-Dimensional Supercritical Fluid Chromatography: Identification of Adequate Chiral Columns and Application to Honey Analysis.

ABSTRACT: Chiral flavonoids are frequently encountered in natural products consumed as food or therapeutic products. However, the taste and other bioactivities of stereoisomers are known to differ. Hence, stereoseparation methods are necessary to resolve these isomers, whether to measure stereoisomeric ratios or to purify individual stereoisomers for activity testing. In this study, we looked for the best conditions to resolve different families of chiral flavonoids, including auronols, flavanonols, aglycon, and glycosylated flavanones. Furthermore, we aimed to include this stereoseparation as the second-dimension method in a two-dimensional supercritical fluid chromatography system (SFC-SFC), to achieve the resolution of chiral flavonoids in complex samples. First, a set of 10 standards of chiral f

SUBMITTER: Reset L 

PROVIDER: S-EPMC12616603 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature