Type IV minor pilin ComN predicted the USS-receptor in Pasteurellaceae.

ABSTRACT: The Uptake Signal Sequence (USS) receptor, which facilitates the acquisition of homologous DNA by natural transformation in Haemophilus influenzae and other members of the Pasteurellaceae, remains unknown. Through discriminating functional gene ontology assessment, cellular localization predictions, and deep-learning structural modeling of protein-DNA complexes, prepilin peptidase-dependent protein A (PpdA) was identified as the strongest USS receptor candidate in different Pasteurellaceae family members with divergent USS specificities. Pasteurellaceae PpdA (PpdA_Past) was the only orthogroup modeled by AlphaFold3 (AF3) to form specific complexes with USS significantly better than complexes with sequence-scrambled versions of USS. Further analyses of PpdA-USS complexes us

SUBMITTER: Helsem SA 

PROVIDER: S-EPMC12616744 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature