Quercetin promotes production of secondary hair follicle stem cells in cashmere goat: a mechanistic study.

ABSTRACT:

Introduction

This study investigated the effects of quercetin on the proliferation and apoptosis of secondary hair follicle stem cells (SHFSCs) isolated from Arbas cashmere goats.

Methods

SHFSCs were treated with varying quercetin concentrations. CCK-8, EdU assays, and flow cytometry analyses were performed to assess cell proliferation and apoptosis. Transcriptome analyses were used to identify differentially expressed genes and enriched signaling pathways.

Results

Treatment with 10 μg/mL quercetin for 48 h significantly promoted cell proliferation. The proportion of S-phase cells increased from 15.5% to 21.2%, and the mRNA and protein levels of PCNA and TERT were upregulated. Quercetin inhibited apoptosis by downregulating BAX, TP53, and CASP3, upregulating BCL2, and

SUBMITTER: Lian W 

PROVIDER: S-EPMC12616863 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature