Cellular osmoregulation enhances porcine embryo development by restoring zygotic genome activation via metabolic-epigenetic crosstalk.

ABSTRACT:

Introduction

In vitro embryo culture is essential for human assisted reproduction and livestock breeding, yet its efficiency remains limited owing to developmental arrest triggered by suboptimal media composition and environmental stressors. Preimplantation embryos are highly sensitive to a minor increase in osmolarity under organic osmolyte deficiency, which disrupts cell volume homeostasis to cause developmental block. However, the osmosensing mechanisms and the causal link between volume dysregulation and developmental arrest remain undefined. Elucidating these mechanisms will identify targeted osmoregulatory interventions to enhance in vitro culture efficiency.

Methods

This study established a porcine two-cell embryo developmental arrest model under physiological-range h

SUBMITTER: Zhao X 

PROVIDER: S-EPMC12616923 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature