Two novel red-FRET ERK bosensors in the 670-720 nm range.

ABSTRACT: Cell fate decisions are regulated by intricate signaling networks, with Extracellular signal-Regulated Kinase (ERK) being a central node in the control of cell proliferation and differentiation. ERK typically cooperates with a network of other regulators, making it necessary to study multiple signaling pathways simultaneously at the single-cell level. Many existing fluorescent biosensors for ERK and other pathways have significant spectral overlap, limiting their utility for multiplexing. To address this limitation, we developed two novel red-FRET ERK biosensors, REKAR67 and REKAR76, which operate in the 670-720 nm range using the fluorescent proteins miRFP670nano3 and miRFP720. REKAR67 and REKAR76 differ in fluorophore position, which impacts biosensor characteristics; REKAR67 displayed a

SUBMITTER: DeCuzzi NL 

PROVIDER: S-EPMC12616953 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature