Transcription fidelity and control of alternative splicing contribute to heat stress survival in Arabidopsis.

ABSTRACT: Transcriptional quality control is essential to maintain the integrity of genetic information. Although well characterized in yeast and metazoans, the regulation of transcriptional fidelity in plants remains elusive. We explored transcriptional fidelity and alternative splicing control in Arabidopsis thaliana using genetic, molecular biology, and deep sequencing tools. Using circle-sequencing assays, we analyzed the error landscape of the transcriptome at single-nucleotide depth under ambient and heat-stress conditions in wild-type and different quality control mutant plants. We found that the frequency of nucleotide misincorporations and insertions is significantly elevated under heat stress, and that nucleotide imbalance also leads to error-prone transcription. We demonstrate that the RN

SUBMITTER: Szaker HM 

PROVIDER: S-EPMC12617145 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature