Systemic neutrophil activation and N-formyl methionine-formyl peptide receptor-1 signaling define inflammatory endotypes in rheumatoid arthritis-associated lung involvement.

ABSTRACT:

Background

Neutrophil activation plays a crucial role in the pathogenesis of rheumatoid arthritis (RA), but its involvement in RA-associated interstitial lung disease (RA-ILD) remains unclear. This study investigated the involvement of N-formyl methionine (fMET) and its receptor formyl peptide receptor-1 (FPR1) in neutrophil-mediated inflammation in RA-ILD.

Methods

Plasma and sputum levels of fMET and neutrophil activation markers were measured by ELISA in two cohorts (n = 269 and 314) spanning multiple disease subgroups. Neutrophil activation was assessed by flow cytometry following plasma stimulation, with or without FPR1 inhibitors.

Results

Calprotectin levels were significantly elevated in both plasma and sputum of RA-ILD patients compared to controls and RA-noILD

SUBMITTER: Shi J 

PROVIDER: S-EPMC12617476 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature