Decellularized lymph node scaffolds accelerate restoration of lymphatic drainage in rat hind limb lymphedema.

ABSTRACT:

Background

There is a lack of effective lymphedema prevention methods. The objective of this study was to investigate the ability of decellularized lymph nodes (dLNs) transplantation to prevent hindlimb lymphedema.

Methods

Porcine dLNs were prepared using 1% sodium dodecyl sulfate and 1% Triton X-100, and the effectiveness of decellularization was assessed by histological assessment and DNA quantification. Lymph node (LN) fragments and dLNs were transplanted into mice, and samples were collected for evaluating biocompatibility at the fourth week postsurgery. Thirty-six SD rats were separated into a control group (lymphatic dissection), a dLNs group (lymphatic dissection and dLNs transplant) and a sham group (inguinal skin circumferentially incised). Hindlimb circumference wa

SUBMITTER: Jian Y 

PROVIDER: S-EPMC12617546 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature