Enhancing peripheral nerve regeneration through NaOH-based decellularization of human nerve tissue.

ABSTRACT: Peripheral nerves are vulnerable to trauma, pressure, and surgical injuries, complicating the regeneration process. While the autograft remains the gold standard for recovery, limitations such as tissue availability and donor site morbidities have led to the exploration of the allografts. However, conventional detergent-based decellularization methods in preparing allografts often cause residual toxicity and damage to the extracellular matrix (ECM). To address such challenges, we propose a sodium hydroxide (NaOH)-based decellularization technique that minimizes harmful residues. Our findings demonstrate that this method effectively removes inflammatory materials while preserving the ECM components and structures, and significantly reduces lipid and detergent residues. In vitro studies conf

SUBMITTER: Kim S 

PROVIDER: S-EPMC12617551 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature