METTL14-mediated m6A modification of DUSP6 mRNA participating in postoperative cognitive dysfunction due to sevoflurane anesthesia.

ABSTRACT:

Background

To investigate the mechanisms underlying sevoflurane-induced POCD, C57BL/6 J mice and SH-SY5Y cells were treated with sevoflurane for model establishment.

Methods

After the treatment with sevoflurane, CCK-8, EdU and flow cytometry were employed to detect cell damage. The levels of N6-methyladenosine (m6A), METTL14 and DUSP6 were determined by qPCR and Western blot. The interaction between METTL14 and DUSP6 was analyzed using RIP-qPCR and Me-RIP methodologies. The cognitive function in mice were assessed by water maze test.

Results

After sevoflurane treatment, the cell viability, cell proliferation and METTL14 expression were markedly suppressed, while apoptosis was significantly enhanced. METTL14 overexpression elevated the levels of m6A and DUSP6, increase

SUBMITTER: Deng S 

PROVIDER: S-EPMC12617634 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature