Subretinal Injection Volume Correlates to Persistent Outer Retinal Thinning in the Pig Eye.

ABSTRACT:

Purpose

Transgenic pig models are a valuable model for preclinical testing of gene and cell therapies. Subretinal injection (SRI) is a common drug delivery method but has been associated with retinal thinning and atrophy. This study examined whether SRI volume correlates with retinal thinning in the pig eye and compared the effects of balanced salt solution (BSS) and adeno-associated virus (AAV) injections.

Methods

Ten eyes from five transgenic pigs were included in this study. Eight eyes received escalating subretinal injection volumes (SRVs) (50, 100, 150, and 200 µL) of either BSS or AAV [5 × 1011 vg/mL], targeting the cone-rich area centralis of the pig eye. After six months, outer retinal thickness inside versus outside the bleb area (ΔORT) was quantified using optical

SUBMITTER: Seitz IP 

PROVIDER: S-EPMC12617665 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature