PRRX1 Orchestrates Pericyte-Myofibroblast Transition in Pathological Retinal Fibrosis.

ABSTRACT:

Purpose

To investigate the role of PRRX1 in pericyte-myofibroblast transition and its contribution to pathological retinal fibrosis.

Methods

Transcriptomic profiling was conducted on human fibrovascular membranes and murine oxygen-induced retinopathy retinas to assess PRRX1 expression and its association with fibrosis-related genes. Single‑cell RNA sequencing was performed on retinal tissues to identify pericyte subpopulations and characterize PRRX1-driven molecular pathways. In vitro, primary mouse retinal pericytes underwent hypoxia with or without small interfering RNA-mediated PRRX1 knockdown to assess fibrotic gene expression and cell migration. In vivo, a laser-induced choroidal neovascularization model was used to assess the effect of PRRX1 silencing on subretinal fib

SUBMITTER: Meng Z 

PROVIDER: S-EPMC12617673 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature