A Triple-Layered Composite Scaffold of Silk Fibroin and Decellularized Amniotic Membrane for Bladder Tissue Engineering.

ABSTRACT: Augmentation cystoplasty has different side effects in urinary bladder reconstruction. Accordingly, it is necessary to develop substitutes using natural and synthetic biomaterials to address current problems. This study evaluates the potential of a triple-layered composite scaffold for bladder regeneration. The triple-layered scaffold consists of a silk fibroin (SF) film blended with polyethylene oxide (PEO), a decellularized human amniotic membrane (DHAM), and a lyophilized SF sponge, which is seeded with adipose tissue-derived stem cells (ADSCs) encapsulated in collagen hydrogel. The mechanical properties of the triple-layered scaffolds closely resemble those of human bladder tissue. The cell survival, proliferation, and viability of the different layers of the scaffold are assessed. The

SUBMITTER: Mamdoohi M 

PROVIDER: S-EPMC12617677 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature