Diagnostic oriented discrimination of different Shiga toxins via PCA-assisted SERS-based plasmonic metasurface.

ABSTRACT: Plasmonic biosensors are powerful platforms for detecting various types of analytes. Specifically, surface-enhanced Raman spectroscopy (SERS) can enable label-free and selective detection. Shiga toxin-producing Escherichia coli (STEC) represents zoonotic pathogens that cause severe diseases, such as hemolytic uremic syndrome (HUS), the most important cause of acute renal failure in children. To date, there are no effective therapies for STEC infection, and the available diagnostic methods are complex and inconclusive. Here, a novel nanopattern fabricated by electron beam lithography with remarkable plasmonic properties is employed as SERS substrate for realizing the specific recognition of Stx1a, Stx2a, and of a third variation of the latter. A limit of detection (LOD) of 6.8 pM for

SUBMITTER: Rippa M 

PROVIDER: S-EPMC12617738 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature