Engineering heterologous core promoters to optimize the SES expression system for <i>Pichia pastoris</i>.

ABSTRACT: Synthetic biology has revolutionized microbial production by broadening the range of substrates, products, and host organisms. Pichia pastoris, a pivotal system for industrial enzyme production and biopharmaceuticals, encounters limitations in production capacity and transcriptional regulation, which impede its wider application. To enhance the performance of P. pastoris, we optimized the synthetic expression system (SES) by engineering heterologous core promoters derived from Trichoderma reesei. Through RNA-seq analysis, we identified high-expression genes' promoters from T. reesei, which were integrated into the SES system to improve its efficiency in P. pastoris. A total of 214 candidate promoters were synthesized and screened based on expression of mC

SUBMITTER: Sun B 

PROVIDER: S-EPMC12617796 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature