Isoflurane anesthesia alters 31P magnetic resonance spectroscopy markers compared to awake mouse brain.

ABSTRACT: Normal brain function hinges on energy-intensive processes. Consequently, alterations to the brain's metabolic state are common hallmarks in several pathological conditions. Phosphorus Magnetic Resonance Spectroscopy (31P MRS) is a noninvasive method for measuring key markers of brain energy metabolism, including adenosine triphosphate (ATP), inorganic phosphate (Pi), and phosphocreatine (PCr), as well as markers for cell membrane phospholipid turnover, phosphomonoester (PME) and phosphodiester (PDE). Preclinical rodent 31P MRS has so far been done under anesthesia - with isoflurane being one of the most commonly used anesthetic agents. The use of isoflurane in 31P MRS is a concern, as anesthetics are known to affect neuronal activity and energy metabolism in the brain. Its use therefore c

SUBMITTER: Molhemi S 

PROVIDER: S-EPMC12617894 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature