Disrupting ribulose-5-phosphate metabolic flux enhances riboflavin production in Escherichia coli BL21(DE3).

ABSTRACT: Riboflavin (vitamin B2) is an essential water-soluble vitamin. To increase its production in a previously engineered strain, R203, we employed metabolic engineering strategies to improve the supply of ribulose-5-phosphate, a key precursor. Disruption of the genes pfkA and edd-eda, which are aimed at promoting ribulose-5-phosphate generation, increased riboflavin production by 51.27% and 65.81%, respectively. To minimize the consumption of ribulose-5-phosphate, we disrupted kdsD and gutQ, both of which encode D-arabinose 5-phosphate isomerase. Only the disruption of gutQ was effective, increasing production by 19.65%, whereas kdsD disruption had no significant effect. Furthermore, disrupting yajO and inserting the pgl gene increased production by 8.65% and 18.80%, respectively. In contrast,

SUBMITTER: Ying J 

PROVIDER: S-EPMC12617950 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature