Establishment of High-Throughput Screening Assay using 384-Well Plate for Identification of Potent Antioxidants from Malaysian Local Plants Repository and Phytochemical Profile of <i>Tetracera Scandens</i>.

ABSTRACT: The increasing prevalence of chronic diseases and oxidative stress-related conditions has led to a growing interest in natural products with potent antioxidant properties. High-throughput screening (HTS) assays offer a promising solution for the rapid evaluation of numerous samples. This study aims to establish a robust HTS assay using the 2-Diphenyl-1-Picrylhydrazyl (DPPH) method to identify natural products with significant antioxidant capabilities, ensuring their safety profiles through cytotoxicity screening and phytochemical profiling. An automated liquid handler with the usage of a 384-well assay plate was employed in optimising and validating the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-HTS assay by calculating the metrics. The HTS metrics include Z-prime (Z') values of 0.72 and 0.63, s

SUBMITTER: Sain AA 

PROVIDER: S-EPMC12618087 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature