Comparison of three multiplex immunoassays for biomarker analysis in stratum corneum tape strips.

ABSTRACT: This study compared three multiplex immunoassay platforms, Meso Scale Discovery (MSD), NULISA, and Olink, for detecting protein markers in stratum corneum tape strips, a non-invasive sampling method challenged by low protein yield. We evaluated 30 shared proteins across all three platforms, plus 9 additional proteins shared only between MSD and NULISA, and 1 between MSD and OLINK, using samples from non-lesional skin and skin affected by patch test-induced irritant and allergic contact dermatitis, and clinical hand dermatitis. Proteins were considered detectable when more than 50% of samples exceeded the platform's protein-specific detection limit. MSD demonstrated the highest sensitivity, detecting 70% of shared proteins, followed by NULISA (30%) and Olink (16.7%). Four proteins, CXCL8, V

SUBMITTER: Metwally NKA 

PROVIDER: S-EPMC12618483 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature