Bidirectional quantitative scattering microscopy.

ABSTRACT: Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are powerful label-free imaging techniques that are widely used in biomedical applications. Each method, however, possesses distinct limitations: QPM, which measures forward scattering (FS), excels at imaging microscale structures but struggles with rapidly moving nanoscale objects, whereas iSCAT, based on backward scattering (BS), is highly sensitive to nanoscale dynamics but lacks the ability to comprehensively image microscale structures. Here, we introduce bidirectional quantitative scattering microscopy (BiQSM), an approach that integrates FS and BS detection using off-axis digital holography with bidirectional illumination and spatial-frequency multiplexing. BiQSM achieves spatiotemporal consistency

SUBMITTER: Horie K 

PROVIDER: S-EPMC12618536 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature