Exploring mitochondrial ribosomal protein S12 as a novel target for non-small cell lung cancer.

ABSTRACT: This study investigates the expression and functional significance of mitochondrial ribosomal protein S12 (MRPS12) within NSCLC pathogenesis. The Cancer Genome Atlas dataset reveals a significant association between MRPS12 overexpression and unfavorable clinical outcomes in NSCLC patients. Single-cell RNA sequencing data unequivocally confirm MRPS12 overexpression across diverse NSCLC cancer cell populations. MRPS12 is also upregulated in locally-treated NSCLC tissues. shRNA-mediated gene silencing or CRISPR-Cas9-mediated gene knockout (KO) of MRPS12 impaired mitochondrial function, resulting in a reduction in oxygen consumption rate, marked declines of complex I activity and ATP levels, mitochondrial membrane depolarization, and an increase in reactive oxygen species production in NSCLC c

SUBMITTER: Chen Z 

PROVIDER: S-EPMC12618574 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature