MiR-5095 inhibits proliferation, migration, and invasion of gastric cancer cells by targeting CEACAM5.

ABSTRACT: Gastric cancer (GC) is a leading cause of cancer-related death, with poor prognosis due to metastasis. Despite improvements in early detection and treatment, effective therapies for metastatic GC are limited. Studies suggest that microRNAs play a key role in GC progression and metastasis. The expression of miR-5095, CEACAM5 and EMT-related proteins were determined by RT-qPCR and western blotting. The effects of miR-5095 on GC cell proliferation were assessed using CCK-8 and EdU assays, while the impact of miR-5095 on GC cell migration and invasion was evaluated using transwell assays. Bioinformatic tools were used to predict potential target genes of miR-5095, and the interaction between miR-5095 and CEACAM5 was confirmed through dual-luciferase reporter assays. Additionally, the expressio

SUBMITTER: Lin W 

PROVIDER: S-EPMC12618673 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature