Exploration of degradation pathways of six antibiotics using a novel Co_0.5Fe_0.5Fe₂O₄ nanozyme.

ABSTRACT: This study investigates the advanced degradation of six common antibiotics using a novel Co_0.5Fe_0.5Fe₂O₄ nanozyme. Under optimal conditions (pH 7, 0.5 mM H₂O₂, 15-min reaction time, and room temperature), the nanozyme demonstrated exceptional catalytic performance, achieved near-complete removal of ciprofloxacin, azithromycin, levofloxacin, moxifloxacin, amoxicillin, and metronidazole. Kinetic studies confirmed its superior catalytic activity, with a Michaelis-Menten constant (K_m) of 0.0366 mM and a maximum reaction velocity (V_max) of 1.10 × 10⁻4 µM·min⁻1. Mass spectrometry (MS) analysis elucidated the degradation pathways by identifying intermediate compounds and stepwise transformations, while total organic carb

SUBMITTER: Ghasemi S 

PROVIDER: S-EPMC12618689 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature