Posterior enhancer (p-Enh) maintains early neuromesodermal progenitors bi-potency during gastrulation.

ABSTRACT: Vertebrate axis patterning requires precise control of the differentiation of neuromesodermal progenitors (NMPs), which generate spinal cord (SC) and presomitic mesoderm (PSM). Previously, we identified a gastrula-premarked posterior enhancer (p-Enh) that is essential for posterior tissue development by regulating somite and SC in organogenetic embryos, while its role in early NMPs cells remains elusive. Here, using a highly efficient in vitro differentiation system, we found that the genetic removal of p-Enh leads to the aberrantly up-regulated PSM-related genes during both PSM and SC differentiation. Time-resolved transcriptomic analysis and experimental characterization revealed the activated PSM transcriptomic signature arose from disorganized NMPs composition, with an over-representat

SUBMITTER: Mi P 

PROVIDER: S-EPMC12618760 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature