Systematic Analysis of SH-SY5Y Differentiation Protocols and Neuronal Subtype Abundance.

ABSTRACT: The SH-SY5Y cell line is a triple-cloned subline of SK-N-SH cells originally isolated in the early 1970s from a bone marrow biopsy of a four-year-old female patient suffering from neuroblastoma. Since then, this cell line has been used as one of the major cell culture models in neuroscience and to study neurodegeneration, as it comprises many of the biochemical and functional properties of neural precursor cells. Differentiation of neuronal precursor cells into a more mature phenotype represents one of the key steps and directed differentiation utilising various reagents is thought to provoke a defined neuronal subtype. Unfortunately, until now there is no consensus, which protocol shall be utilised to reach a specific neuronal subtype. Thus, the aim of the present work was to evaluate fou

SUBMITTER: Prisacar M 

PROVIDER: S-EPMC12618781 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature