Evaluation and validation of reference genes for RT-qPCR normalization in different sweet potato tissues.

ABSTRACT: Sweet potato (Ipomoea batatas) is one of the most important crops of the world, displaying a significant economic importance in several countries. However, few studies exploring biotechnological tools, such as RT-qPCR, used for gene expression analysis, have been conducted so far, slowing down crop breeding programs. Here, a detailed analysis of previous validated reference genes, essential for normalization of RT-qPCR studies, was conducted, and new candidate genes were evaluated, addressing existing gaps for the development of molecular studies via RT-qPCR in sweet potato. To this end, five sweet potato reference genes studies were evaluated, and the six best-classified genes (IbCYC, IbARF, IbTUB, IbUBI, IbCOX and IbEF1α) were selected for further analysis. Additionally, four commonly us

SUBMITTER: Moraes MBF 

PROVIDER: S-EPMC12618874 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature