MiR-629-3p affects postmenopausal osteoporosis through targeted regulation of BACH1.

ABSTRACT:

Background

Osteoporosis (OP) represents a major global health challenge, particularly among postmenopausal women. Currently, there remains a shortage of reliable diagnostic biomarkers and targeted therapies for OP.

Methods

A cohort of 254 postmenopausal women (127 OP patients and 127 healthy controls) was analyzed. Serum miR-629-3p levels were measured via qRT-PCR, and diagnostic potential was assessed using ROC analysis. In vitro, MC3T3-E1 osteoblasts were transfected with miR-629-3p mimic/inhibitor and BACH1 overexpression vector (oe-BACH1). Proliferation and apoptosis were evaluated by functional assays (CCK-8, Annexin V-FITC/PI staining), while osteogenic markers (FN1, ITGB1 and RUNX2) were assessed by qPCR. The binding of miR-629-3p to the BACH1 3'UTR was confirmed by l

SUBMITTER: Yun D 

PROVIDER: S-EPMC12619170 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature