On-line identification of chemical constituents in Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata based on UHPLC-Q-Orbitrap-Linear Ion Trap-MS.

ABSTRACT:

Objective

To rapidly characterize the chemical constituents of Polygoni Multiflori Radix and its processed form, Polygoni Multiflori Radix Preparata, utilizing ultra-high performance liquid chromatography coupled with quadrupole-orbitrap-linear ion trap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-Linear Ion Trap-MS).

Methods

Chromatographic separation was achieved on an ACQUITY UPLC BEH phenyl column (1.7 μm, 2.1 × 100 mm) employing a gradient elution of methanol (A) and 0.1% formic acid aqueous solution (B). Mass spectrometric analysis was conducted using a heated electrospray ionization (H-ESI) source operated in negative ion mode. Data processing was performed using Thermo Scientific Compound Discoverer 3.0 software, integrating a self-constructed Polygoni Multifl

SUBMITTER: Yao R 

PROVIDER: S-EPMC12619182 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature