Characteristics of extracellular vesicle-derived lncRNAs during the progression of HBV-related hepatocellular carcinoma.

ABSTRACT: Liver diseases, including hepatitis B virus (HBV) infection, cirrhosis, and hepatocellular carcinoma (HCC), represent significant global health challenges with limited non-invasive biomarkers for early detection. Extracellular vesicles (EVs), enriched with disease-specific RNAs, offer a promising avenue for biomarker discovery. This study characterized EV-derived long non-coding RNAs (lncRNAs) across various stages of liver disease and explored their mechanistic roles. Serum EVs were isolated from 24 participants (5 healthy controls, 5 chronic hepatitis B patients, 5 liver cirrhosis patients, 4 hepatocellular adenoma patients, and 5 HCC patients) using ultracentrifugation and validated by transmission electron microscopy, nanoparticle tracking analysis, and Western blot. High-throughput tr

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12619285 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature