Immunoinformatics-based strategies for developing DNA and mRNA vaccines against monkeypox virus (MPXV).

ABSTRACT: The monkeypox virus (MPXV) is a newly discovered zoonotic orthopoxvirus that can infect humans and shares similarities with the smallpox virus. With no clinically validated treatment for MPXV infections, it is important to develop a broad-range vaccine that is effective against this disease. This study aimed to design novel multiple-epitope DNA and mRNA vaccines against MXPV using comprehensive immunoinformatics and reverse vaccinology techniques. Eleven MPXV proteins were selected from the UniProt database and assessed for their antigenicity and allergenicity. Proteins exhibiting significant antigenicity and non-allergenic characteristics were examined for the prediction of T-cell and B-cell epitopes. Four MHC-I, eight MHC-II, and six B-cell epitopes were coupled with specific linkers and

SUBMITTER: Khan S 

PROVIDER: S-EPMC12619427 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature