<i>Minos</i>-mediated transgenesis in the pantry moth <i>Plodia interpunctella</i>.

ABSTRACT: Transposon-mediated transgenesis has been widely used to study gene function in Lepidoptera, with piggyBac being the most commonly employed system. However, because the piggyBac transposase originates from a lepidopteran genome, it raises concerns about endogenous activation, remobilization, and silencing of transgenes, thus questioning its suitability as an optimal tool in Lepidoptera. As an alternative, we evaluated the dipteran-derived Minos transposase for stable germline transformation in the pantry moth, Plodia interpunctella. We injected syncytial embryos with transposase mRNA, along with donor plasmids encoding 3xP3::EGFP and 3xP3::mCherry markers of eye and glial tissues. Across multiple experiments, we found that G₀ injectees c

SUBMITTER: Shodja DN 

PROVIDER: S-EPMC12619579 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature