Advancing Chemoenzymatic Synthesis and Covalent Immobilization of a Comprehensive Ganglio-glycosphingolipid Library Enables Functional Multiplex Bead Assays.

ABSTRACT: Ganglio-series glycosphingolipids (ganglio-GSLs) are biologically important glycolipids comprising a glycan of a ganglio-series nature and a lipid called ceramide. The glycan component often contains one or more sialic acid residues. Accesses to structurally defined ganglio-GSLs remain limited due to their structural complexity and synthetic challenges, hindering both functional studies and therapeutic applications. To overcome the challenges, we report herein a scalable chemoenzymatic total synthesis strategy for the construction of the most comprehensive library of ganglio-GSLs to date. A chiral pool approach was developed for synthesizing simple glycosylsphingosines with desired sphingosine lengths (d18:1 or d20:1) from inexpensive d-xylose. Complex glycosylsphingosines were prepared us

SUBMITTER: Gucchait A 

PROVIDER: S-EPMC12619687 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature